Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration
Identifieur interne : 003951 ( Main/Exploration ); précédent : 003950; suivant : 003952Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration
Auteurs : C. Scottez [France] ; E. Chevreau [France] ; N. Godard [France] ; Y. Arnaud [France] ; M. Duron [France] ; J. Dereuddre [France]Source :
- Cryobiology [ 0011-2240 ] ; 1992.
Abstract
Cryopreservation of axillary shoot-tips of pear in vitro cultures (Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75 M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).
Url:
DOI: 10.1016/0011-2240(92)90073-B
Affiliations:
- France
- Languedoc-Roussillon, Occitanie (région administrative), Pays de la Loire, Île-de-France
- Beaucouzé, Bellegarde, Paris
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000B18
- to stream Istex, to step Curation: 000B18
- to stream Istex, to step Checkpoint: 001108
- to stream Main, to step Merge: 003C59
- to stream Main, to step Curation: 003951
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title><author><name sortKey="Scottez, C" sort="Scottez, C" uniqKey="Scottez C" first="C." last="Scottez">C. Scottez</name></author><author><name sortKey="Chevreau, E" sort="Chevreau, E" uniqKey="Chevreau E" first="E." last="Chevreau">E. Chevreau</name></author><author><name sortKey="Godard, N" sort="Godard, N" uniqKey="Godard N" first="N." last="Godard">N. Godard</name></author><author><name sortKey="Arnaud, Y" sort="Arnaud, Y" uniqKey="Arnaud Y" first="Y." last="Arnaud">Y. Arnaud</name></author><author><name sortKey="Duron, M" sort="Duron, M" uniqKey="Duron M" first="M." last="Duron">M. Duron</name></author><author><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/0011-2240(92)90073-B</idno><idno type="url">https://api.istex.fr/document/C7F3C19F622F8EBEB15762FDF36445AA49922746/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B18</idno><idno type="wicri:Area/Istex/Curation">000B18</idno><idno type="wicri:Area/Istex/Checkpoint">001108</idno><idno type="wicri:doubleKey">0011-2240:1992:Scottez C:cryopreservation:of:cold</idno><idno type="wicri:Area/Main/Merge">003C59</idno><idno type="wicri:Area/Main/Curation">003951</idno><idno type="wicri:Area/Main/Exploration">003951</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title><author><name sortKey="Scottez, C" sort="Scottez, C" uniqKey="Scottez C" first="C." last="Scottez">C. Scottez</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Chevreau, E" sort="Chevreau, E" uniqKey="Chevreau E" first="E." last="Chevreau">E. Chevreau</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CTIFL, Centre de Balandran, F 30127 Bellegarde</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Bellegarde</settlement></placeName></affiliation></author><author><name sortKey="Godard, N" sort="Godard, N" uniqKey="Godard N" first="N." last="Godard">N. Godard</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Arnaud, Y" sort="Arnaud, Y" uniqKey="Arnaud Y" first="Y." last="Arnaud">Y. Arnaud</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie du Développement des Plantes, Université Paris VI, F 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Duron, M" sort="Duron, M" uniqKey="Duron M" first="M." last="Duron">M. Duron</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, F 49070 Beaucouzé</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region><settlement type="city">Beaucouzé</settlement></placeName></affiliation></author><author><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Cryobiologie Végétale Université Paris VI, F 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Cryobiology</title><title level="j" type="abbrev">YCRYO</title><idno type="ISSN">0011-2240</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="691">691</biblScope><biblScope unit="page" to="700">700</biblScope></imprint><idno type="ISSN">0011-2240</idno></series><idno type="istex">C7F3C19F622F8EBEB15762FDF36445AA49922746</idno><idno type="DOI">10.1016/0011-2240(92)90073-B</idno><idno type="PII">0011-2240(92)90073-B</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0011-2240</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cryopreservation of axillary shoot-tips of pear in vitro cultures (Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75 M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li><li>Pays de la Loire</li><li>Île-de-France</li></region><settlement><li>Beaucouzé</li><li>Bellegarde</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Scottez, C" sort="Scottez, C" uniqKey="Scottez C" first="C." last="Scottez">C. Scottez</name></region><name sortKey="Arnaud, Y" sort="Arnaud, Y" uniqKey="Arnaud Y" first="Y." last="Arnaud">Y. Arnaud</name><name sortKey="Chevreau, E" sort="Chevreau, E" uniqKey="Chevreau E" first="E." last="Chevreau">E. Chevreau</name><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name><name sortKey="Duron, M" sort="Duron, M" uniqKey="Duron M" first="M." last="Duron">M. Duron</name><name sortKey="Godard, N" sort="Godard, N" uniqKey="Godard N" first="N." last="Godard">N. Godard</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003951 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003951 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:C7F3C19F622F8EBEB15762FDF36445AA49922746
|texte= Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration
}}
| This area was generated with Dilib version V0.6.25. |  |